QF-Pro® Reagent Kits
Realise the potential of QF-Pro® with our flexible reagent kits
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- QF-Pro® Reagent Kits
Launching now are our proprietary QF-Pro® reagent kits.
Our QF-Pro® kits contain the tailored secondary detection reagents and specific components necessary to perform functional protein labelling in situ.
It includes secondary antibodies for FRET, quenching and blocking buffers as well as the signal amplification reagents. These kits are designed to allow the users to test their favourite primary antibodies for the detection of protein function in FFPE, tissue and cells.
All QF-Pro® reagent kits will be stable for at least 6 months upon receipt and contain enough reagents to perform QF-Pro® labelling on up to 100 FFPE tissue slides or a 96 -well plate
How does QF-Pro® work?
QF-Pro® has modified established technologies (FRET/FLIM) to create a patented assay which is able to spatially quantify protein functions in situ within FPPE tissue samples for the first time at an unprecedented resolution. This is a feat that is unachievable with existing technologies.
QF-Pro® utilises a two-site immunofluorescence-styled assay in conjunction with amplified Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM). Essentially operating as a “biochemical ruler”, QF-Pro® makes 1-10nm measurements within samples to quantify protein functional states. Our patented amplification step has allowed these readouts to perform in patient tissue samples, a feat that has been unachievable with previous FRET-based technologies.
Two epitopes are labelled simultaneously using species-distinct antibodies. These are then detected with QF-Pro® secondary labelling reagents, one combined with a green chromophore (the donor) and the other to an amplified red chromophore (the acceptor).
Violet 3.0 and our QF-Pro® software then automatically measures the distance between the donor and acceptor chromophore, based on the amount of energy given from the donor to the acceptor (this is FRET). This allows us to quantify the interaction or post-translational modification of a protein and spatially measure these events across a sample. Violet 3.0 makes FRET imaging easier and more accessible than ever before.
Violet 3.0
All-in-one microscopy platform for QF-Pro® technology, bringing Fluorescence Lifetime Imaging Microscopy (FLIM) and Förster Resonance Energy Transfer (FRET) to any lab
Publications
Read latest manuscripts to see how QF-Pro® has been used to advance translational and clinical research.
